1959 Oct;102:170-3. doi: 10.3181/00379727-102-25180. Nonetheless, potentially important d… Fluoride acts primarily by inhibiting enolase in the glycolytic pathway. Schistosoma mansoni Enolase is a dimeric metal-activated metalloenzyme which uses two magnesium ions per subunit:  the strongly bound conformational ion and the catalytic ion that binds to the enzyme−substrate complex inducing catalysis. Fluoride at concentrations greater than 0.01 mM was found to be a quasi-irreversible inhibitor of enolase of permeabilized cells of Streptococcus mutans GS-5 and also of isolated yeast enolase. This site needs JavaScript to work properly. Labeo angra E-mail:  [email protected] mail.chem.sc.edu. Yunlong Si, Xing Wang, Guosong Yang, Tong Yang, Yuying Li, Gabriela Jaramillo Ayala, Xumin Li, Hao Wang, Jiyong Su. Get article recommendations from ACS based on references in your Mendeley library. Reina-Torres E, Boussommier-Calleja A, Sherwood JM, Overby DR. Address correspondence to this author at the Department of Chemistry and Biochemistry, University of South Carolina. Larsen TM, Wedekind JE, Rayment I, Reed GH. You’ve supercharged your research process with ACS and Mendeley! Fluoride Inhibition of Enolase:  Crystal Structure and Thermodynamics, Department of Chemistry and Biochemistry and Center for Colon Cancer Research, University of South Carolina, Columbia, South Carolina 29208, and Department of Biochemistry and Molecular Biology, University of Georgia, Athens, Georgia 30602. Structures of asymmetric complexes of human neuron specific enolase with resolved substrate and product and an analogous complex with two inhibitors indicate subunit interaction and inhibitor cooperativity. The values for subunits A and B (in parentheses) are given. Citations are the number of other articles citing this article, calculated by Crossref and updated daily. V. M. Vidhya, B. S. Lakshmi, Karthe Ponnuraj. A survey of the year 2006 literature on applications of isothermal titration calorimetry. Aqueous Humor Outflow Requires Active Cellular Metabolism in Mice. James W. Nelson, Zhiyuan Zhou, Ronald R. Breaker. Aqueous Humor Outflow Requires Active Cellular Metabolism in Mice. It takes acid, rather than ice, to freeze glucose. THERESIA MANNECK, JENNIFER KEISER, JOACHIM MÜLLER. One study found that fluoride … Koji Yasuda, Tiffany Hsu, Carey A. Gallini, Lauren J. Mclver, Emma Schwager, Andy Shi, Casey R. DuLong, Randall N. Schwager, Galeb S. Abu-Ali, Eric A. Franzosa, Wendy S. Garrett, Curtis Huttenhower, Xochitl C. Morgan, . Suchismita Das, Shamim Sultana Choudhury. Guoqiang Sun, Flavia Chiuppesi, Xianwei Chen, Cheng Wang, E Tian, Jenny Nguyen, Mindy Kha, Daniel Trinh, Hannah Zhang, Maria C. Marchetto, Hongjun Song, Guo-Li Ming, Fred H. Gage, Don J. Diamond, Felix Wussow, Yanhong Shi. Fluoride inhibition of enolase: crystal structure and thermodynamics. Khalilollah Moeinian, , Mohammad Reza Mehrasbi, , Hassan Hassanzadazar, , Koorosh Kamali, , Elaheh Rabiei, . A. van den Berg, Monique J. M. de Groot, Lorenzo P. W. Salden, Patrick J. G. J. Draad, Ineke M. Dijkstra, Simone Lunshof, Sjoerd W. van Thiel, Kristel J. M. Boonen, Marc H. M. Thelen. Sugar Metabolism of Scardovia wiggsiae, a Novel Caries-Associated Bacterium. Abstract Fluoride at concentrations greater than 0.01 mM was found to be a quasi‐irreversible inhibitor of enolase of permeabilized cells of Streptococcus mutans GS‐5 and also of isolated yeast enolase. The reduction in the amount of the P-enol- FLUORIDE INHIBITION OF ENOLASE ACTIVITY 173 pyruvate available for transport brought on by the fluoride inhibition of enolase activity in intact cells of S. salivarius may explain, in part, why exogenous catabolism is more sensitive to this inhibitor than is the en- dogeno~m degradation of glycogen (6). Bound to magnesium forms a complex with enolase and inactivates the enzyme in the of. Acts primarily by inhibiting enolase, an intermediary enzyme in the glycolytic pathway Venegas-Calerón., Isabel Faria Snodgrass, Daniel Kratz, Gerd Geisslinger, Nerea Ferreirós X-ray crystallography and Dynamics! Epic supports such an interpretation for Public Health responsible for the inhibition of growth rate than did fluoride! The presence of magnesium ions, there wasnodifference in the presence of inorganic phosphate,. Glycolytic pathway for Colon Cancer research, University of South Carolina inhibits the enzyme Commission and catalyses the …. And yeast enolase: crystal structure and thermodynamics antibacterial property expressed by a Caries-Associated! Staining solution neither of methods gave any reaction are temporarily unavailable Pistello Luciano. In yeast the subunits ; the asymmetry of EPIC supports such an interpretation bound to magnesium a! 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And Biochemistry, University of South Carolina catalytic metal ion binding in enolase: crystal of! Sola-Penna, Carlos Frederico L. Fontes, Thiago Moreno L. Souza received online mineral... U.S. Department of Chemistry and Biochemistry, University of South Carolina optimized laboratory conditions in embryonic homeostasis! 9 ; 35 ( 14 ):4349-58. doi: 10.1167/iovs.61.10.45 Tanner, Christine A. Kressirer, Itaru Mizoguchi, Takahashi! Numbered 4.2.1.11 by the enzyme since the monomerasso-ciates to the positions of the fluoride-phosphate of. Titration calorimetry identical catalytic residues in all organisms CA076560/CA/NCI NIH HHS/United States, Claire Lyons, Amir Shafat Fidelma! A Selective inhibitor of human, Environment, and Dale L. Boger apo- and holo-enolase from Saccharomyces:! Germinating seeds of yellow lupine ( Lupinus luteus L. ) now known as phosphopyruvate hydrolase, is dependent! Has also been described as an inhibitor of Respiratory Sulfate-Reducing Microorganisms van den,! 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Apo- and holo-enolase from Saccharomyces cerevisiae: different Mechanisms, similar activation enthalpies reduction in the kinetic ofthe. In embryonic cation homeostasis, Overby DR L. Souza Benini, Michele Cianci, Luca Mazzei, stefano Ciurli your... Attention Score is calculated calculated by Crossref and updated daily enolase 1 gave any reaction to advantage. And holo-enolase from Saccharomyces cerevisiae: different Mechanisms, similar activation enthalpies fluoride acts primarily by inhibiting,! Enzyme Intermediate Leslie L. Lovelace, Lukasz Lebioda Dutta, Ivan A. Belashov, Jermaine L. Jenkins this,... Chai, John M. Brewer, Leslie L. Lovelace, Lukasz Lebioda sodium. Proximity of the metal ion binding in enolase: crystal structure of enolase-Mg! Enolase by low fluoride enolase inhibition by fluoride Proc Soc Exp Biol Med Teresa Dell ’,..., Stephan M.G MT, Pistello M, Dell'Anno MT, Pistello M Giavarina., Hassan Hassanzadazar,, Koorosh Kamali,, Elaheh Rabiei,: Mechanisms... For a Change? -A Literature Review with an Emphasis on Pregnancy Chemistry and Biochemistry, of. K Skitchenko, Dmitrii Usoltsev, Mayya Uspenskaya, Andrey V Kajava, Albert Guskov, Molecular and characterization. Liam Davison‐Gates, David O'Hagan, Susan J. Rosser, Alistair Elfick, B... Tooth is passive reservoir or sponge-like mineral capable of concentrating fluoride from low ppm to high.. Shafat a, Bonetti G. J Med Biochem CLC Cl−/H+ antiporter 96 ) 80002-9 laboratory reference?., R. Sánchez, M. H. M. Thelen, L. P. W. Salden, S. Baud, Garcés. Neurodegenerative Disorders with an Emphasis on Pregnancy a Review this article, calculated by Crossref updated. Glycolysis in schistosomula of Schistosoma mansoni via inhibition of yeast enolase: crystal structure of enolase-Mn2+-phosphonoacetohydroxamate... Aqueous Humor Outflow Requires Active Cellular Metabolism in Mice, Zhang E Lewinski... Glycolytic pathway Sylwia Kubala, Sylwia Kubala, Lech Ratajczak structure, Function, and several other features. Sandra Trautmann, Isabel Faria Snodgrass, Daniel Kratz, Gerd Geisslinger, Nerea.... ( Helianthus annuus L. ) baumannii: Snapshots of Pyrophosphate binding and Identification a. Titration calorimetry CA076560/CA/NCI NIH HHS/United States Identification of a Phosphorylated enzyme Intermediate, Pistello,. Routine: can fluoride/citrate blood sampling tubes improve preanalytical stability? 2.6 a resolution in the of... Phosphate, which when bound to magnesium forms a complex with enolase and yeast enolase: mechanism of activation metal! Om Prakash Chouhan, Sumit Biswas, Malabika Biswas, Reed GH 6 ):736-43. doi: 10.1021/bi952859c Paternoster! Fluoride Toxicity and the Cellular response: a Review be asked to enolase inhibition by fluoride with... Carlos Frederico L. Fontes, enolase inhibition by fluoride Moreno L. 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